However, the SD and HRE of the LTC-IC frequencies were still substantial (about 25%) even when 96 wells were seeded per dilution. 1) that increasing the number of replica indeed decreases the uncertainty of the LTC-IC measurements. ![]() ![]() We reanalyzed the LTC-IC dataset, taking into account the number of wells that were seeded per cell concentration. The original dataset contained experiments that were performed with different numbers of replica. Statistical considerations predict that increasing the number of replica for each determination will improve the precision of the data. We wished to determine which errors contribute to this uncertainty and whether the precision of the measurement could be improved. Despite the large number of experiments, the estimate of LTC-IC frequency has a large uncertainty with a coefficient of variation (CV), Fig. ], the mean frequency of LTC-IC in this strain of mice is 2.78 ± 1.06 ( Table 1).
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